Download e-book for kindle: Antibody Engineering Protocols by Sudhir Paul

By Sudhir Paul

ISBN-10: 0896032752

ISBN-13: 9780896032750

This complete selection of lately constructed equipment for generating new antibody reagents by means of immunization and recombinant DNA recommendations comprises ready-to-use protocols that light up present components of analysis on antibody constitution, services, and functions. The tools may be utilized in uncomplicated immunological stories concerning antibody specificity, catalysis, and evolution, and within the isolation of infrequent antibodies through phage demonstrate expertise and the engineering of recent antibodies via mutagenesis. they provide perception into new methods of constructing clinically worthy antibody reagents. Antibody Engineering Protocols constitutes a single-source quantity for laboratory investigators who are looking to reduce vast literature and method searches and to paintings productively of their fields with reproducible step by step protocols.

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Proterns: Struct. Funct. Genet. 6,382-394. 46. , and Lesk, A. M. J. Mol. Biol. , Lesk, A. , Tomlinson, I. , Llewelyn, M. , and Winter, G. (1992) Structural repertoire of the human x segments. J. Mol. Biol 227,799-817. 48 Tramontano, A and Lesk, A M. (1992) Common features of the conformations of antigen-bmdmg loops in immunoglobulins and application to modeling loop conformations. Proteins Struct. Funct Genet. 13,23 l-245 49 Wu, S and Cygler, M (1993) Conformation of complementarity determining region Ll loop m murine IgG A light chain extends the repertoire of canonical forms J Mol.

Biol. 210,601-615 114. Herron, J. , He, X. , Mason, M. , Voss, E. , and Edmundson, A. B. (1989) Three-dimensional structure of a fluorescein Fab complex crystallized in 2-methyl-2,4pentanediol. Proteins: Struct. Funct. Genet. $271-280. 115. Rose, D. , Margolies, M. , Gefter, M. , and Petsko, G. A. (1990) Crystal structure of the antigen-binding fragment of the murine antiarsonate monoclonal antibody 36-71 at 2 9 8, resolution. Proc. Natl. Acad. Sci USA 87,338-342. 116. Saul, F. A. and Poljak, R.

And Davies, D. R. (1989) Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. Proc. Natl. Acad. Sci. USA 86, 5938-5942 113 Ely, K. , Herron, J. , and Edmundson, A. B. (1989) Three-dimensional structure of a light chain dimer crystallized in water. J. Mol. Biol. 210,601-615 114. Herron, J. , He, X. , Mason, M. , Voss, E. , and Edmundson, A. B. (1989) Three-dimensional structure of a fluorescein Fab complex crystallized in 2-methyl-2,4pentanediol. Proteins: Struct.

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Antibody Engineering Protocols by Sudhir Paul

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