By John R. Masters (Editor)
This re-creation of Animal telephone tradition covers new or up-to-date chapters on telephone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem cellphone assays, three-dimensional tradition, tissue engineering and cytotoxicity assays. specified protocols for a large choice of tools give you the center of every bankruptcy, making new method simply available. every body operating in organic and clinical examine, no matter if in academia or a advertisement association, training cellphone tradition will profit vastly from this e-book.
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Additional info for Animal Cell Culture: A Practical Approach 3rd Edition
And Newall, D. G. ) (1990). Cell and tissue culture: laboratory procedures. Wiley, Chichester. 2. Davis, J. M. ) (1994). Basic cell culture: a practical approach. IRL Press at Oxford University Press, Oxford. 3. Freshney, R. I. (2000). Culture of animal cells: a manual of basic technique, 4th edn. WileyLiss, New York. 4. Freshney, R. I. (1999). Freshney's culture of animal cells, a multimedia guide. Wiley-Iiss, New York. 5. Boyce, S. T. and Ham, R. G. (1983) J. Invest. Dermatol, 81 (Suppl 1), 33s.
6. 2 Comparison of batch, perfusion, and continuous-flow culture (Figure 2) Continuous-flow culture is the only system in which the cellular content is homogeneous, and can be kept homogeneous for long periods of time (months). This can be vital for physiological studies, but may not be the most economical method for product generation. Production economics are calculated in terms of staff time, medium, equipment, and downstream processing costs. Also taken into account are the complexity and sophistication of the equipment and process, as this affects the calibre of the staff required and the reliability of the production process.
Thus, glucose should ideally never be included in media at concentrations above 2 g/litre, and it is better to supplement during the culture than to increase the initial concentration. An alternative is to substitute glucose by galactose or fructose as this significantly reduces the formation of lactic acid, but it usually results in a slower growth rate. These precautions delay the onset of a nonphysiological pH and are sufficient for small cultures. As scale-up increases, headspace volume and culture surface area in relation to the medium volume decrease.
Animal Cell Culture: A Practical Approach 3rd Edition by John R. Masters (Editor)