Get An Introduction to Genetic Engineering (Studies in Biology) PDF

By Dr Desmond S. T. Nicholl

ISBN-10: 0521004713

ISBN-13: 9780521004718

Des Nicholl offers a brand new, absolutely revised, and improved version of his renowned undergraduate-level textbook. The publication keeps a number of the good points of the unique version and nonetheless deals a concise technical advent to the topic of genetic engineering. it really is divided into 3 major sections: uncomplicated molecular biology, equipment of gene manipulation, and smooth purposes of genetic engineering. purposes lined within the ebook contain genomics, protein engineering, gene treatment, cloning, transgenic animals and vegetation, and bioethics. An advent to Genetic Engineering is vital examining for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.

Show description

Read or Download An Introduction to Genetic Engineering (Studies in Biology) PDF

Similar molecular biology books

Douglas C. Rees (Eds.)'s Membrane Proteins PDF

This quantity covers 2 significant issues: Foundations and Membrane Protein constructions. Key positive aspects * Foundations * Bioenergetic procedures * Channels and Receptors

Basic Cell Culture - download pdf or read online

During this totally revised variation of a longtime vintage, specialist researchers and clinicians describe in step by step aspect up-to-date concepts for the isolation and development of significant fundamental phone varieties, similar to kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors provide with no trouble reproducible new tools for the differentiation of embryonic stem (ES) cells into a variety of hematopoietic telephone forms, for fetal thymic organ tradition, and for the isolation and tradition of specialised mobile varieties, similar to mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal phone traces, and endothelial cells.

Download e-book for kindle: Biophysical, Chemical and Functional Probes of RNA by Daniel Herschlag

This MIE quantity presents laboratory suggestions that goal to foretell the constitution of a protein that can have super implications starting from drug layout, to mobile pathways and their dynamics, to viral access into cells. specialist researchers introduce the main complex applied sciences and methods in protein constitution and foldingIncludes options on tiling assays.

Glucose Homeostatis and the Pathogenesis of Diabetes by Ya-Xiong Tao PDF

Diabetes mellitus is a illness with super healthiness and fiscal burden. a greater realizing of ways basic glucose homeostasis is maintained and the pathogenesis is critical to spot new methods for diabetes remedy. This publication addresses a number of facets of this sector of analysis. Key good points: * Written by means of specialists within the box * Informs on very important issues on the topic of the rules of glucose homeostasis and the pathogenesis of diabetes mellitus, a box of severe learn curiosity

Additional info for An Introduction to Genetic Engineering (Studies in Biology)

Sample text

To prevent the enzyme acting on the host cell DNA, the modification enzyme of the system (a methylase) modifies the host DNA by methylation of particular bases in the recognition sequence, which prevents the restriction enzyme from cutting the DNA. Restriction enzymes are of three types (I, II or III). Most of the enzymes used today are type II enzymes, which have the simplest mode of action. 1), and as they cut at an internal position in a DNA strand (as opposed to beginning degradation at one end) they are known as endonucleases.

In this way nanogram or picogram amounts may be dispensed with reasonable accuracy. 30 Working with nucleic acids Precipitation of nucleic acids is an essential technique that is used in a variety of applications. The two most commonly used precipitants are isopropanol and ethanol, ethanol being the preferred choice for most applications. 2 M salt, ethanol causes the nucleic acids to come out of solution. Although it used to be thought that low temperatures (Ϫ20°C or Ϫ70°C) were necessary, this is not an absolute requirement, and 0 °C appears to be adequate.

Transcription and translation. (a) Transcription involves synthesis of mRNA by RNA polymerase. Part of the DNA/mRNA sequence is given. The mRNA has the same sequence as the coding strand in the DNA (the non-template strand), apart from U being substituted for T. (b) The ribosome is the site of translation, and is made up of the large subunit (LSU) and the small subunit (SSU), each made up of ribosomal RNA molecules and many different proteins. There are three sites within the ribosome. The A (aminoacyl) and P (peptidyl) sites are involved in insertion of the correct tRNA–amino acid complex in the growing polypeptide chain.

Download PDF sample

An Introduction to Genetic Engineering (Studies in Biology) by Dr Desmond S. T. Nicholl


by Paul
4.5

Rated 4.94 of 5 – based on 39 votes