By Dr Desmond S. T. Nicholl
Des Nicholl offers a brand new, absolutely revised, and improved version of his renowned undergraduate-level textbook. The publication keeps a number of the good points of the unique version and nonetheless deals a concise technical advent to the topic of genetic engineering. it really is divided into 3 major sections: uncomplicated molecular biology, equipment of gene manipulation, and smooth purposes of genetic engineering. purposes lined within the ebook contain genomics, protein engineering, gene treatment, cloning, transgenic animals and vegetation, and bioethics. An advent to Genetic Engineering is vital examining for undergraduate scholars of biotechnology, genetics, molecular biology, and biochemistry.
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Additional info for An Introduction to Genetic Engineering (Studies in Biology)
To prevent the enzyme acting on the host cell DNA, the modiﬁcation enzyme of the system (a methylase) modiﬁes the host DNA by methylation of particular bases in the recognition sequence, which prevents the restriction enzyme from cutting the DNA. Restriction enzymes are of three types (I, II or III). Most of the enzymes used today are type II enzymes, which have the simplest mode of action. 1), and as they cut at an internal position in a DNA strand (as opposed to beginning degradation at one end) they are known as endonucleases.
In this way nanogram or picogram amounts may be dispensed with reasonable accuracy. 30 Working with nucleic acids Precipitation of nucleic acids is an essential technique that is used in a variety of applications. The two most commonly used precipitants are isopropanol and ethanol, ethanol being the preferred choice for most applications. 2 M salt, ethanol causes the nucleic acids to come out of solution. Although it used to be thought that low temperatures (Ϫ20°C or Ϫ70°C) were necessary, this is not an absolute requirement, and 0 °C appears to be adequate.
Transcription and translation. (a) Transcription involves synthesis of mRNA by RNA polymerase. Part of the DNA/mRNA sequence is given. The mRNA has the same sequence as the coding strand in the DNA (the non-template strand), apart from U being substituted for T. (b) The ribosome is the site of translation, and is made up of the large subunit (LSU) and the small subunit (SSU), each made up of ribosomal RNA molecules and many different proteins. There are three sites within the ribosome. The A (aminoacyl) and P (peptidyl) sites are involved in insertion of the correct tRNA–amino acid complex in the growing polypeptide chain.
An Introduction to Genetic Engineering (Studies in Biology) by Dr Desmond S. T. Nicholl